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Molecular Diagnostics. Protein Analysis. Applied Sciences. Drug Discovery. Featured Research Topics. Infectious Diseases. Custom Capabilities. Onsite Stocking. Format and QC. Automation Solutions. Custom Assay Development. Student Resources. Peer Reviewed Literature. Product Usage Information. Global Support. Medical Affairs. Local Sales Support. About Promega. Join Our Team. Contact Us. Your Cart. Current Items 0. Catalog number selected: D Together, these preformulated, high-quality buffers cover a spectrum of PCR performance capabilities for endpoint, multiplex, real-time, GC-rich and inhibitor-resistant amplifications.
These products are general purpose reagents intended for general laboratory use. They can be used as a component of molecular diagnostic assays where applicable country laws allow.
Separate license or royalty payments are not required. Search by lot number. Optimized, dependable reverse transcription. Available as kit, master mix or standalone enzyme. The most-trusted product for broad-spectrum inhibition of common eukaryotic RNases. We use these cookies to ensure our site functions securely and properly; they are necessary for our services to function and cannot be switched off in our systems. They are usually only set in response to actions made by you which amount to a request for services, such as logging in, using a shopping cart or filling in forms.
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Assay Set-Up: Before starting, vortex all components thoroughly to ensure homogeneity. Prepare a premix for the number of assays you need according to the following protocol: comp. Crystal Buffer PCR is recommended for down-stream applications such as DNA sequencing, ligation, restriction digestion or where an analysis of the PCR product by absorbance or fluorescence excitation is required.
For gel electrophoresis add gel loading buffer and fluorescent DNA stain i. Using pre-stained gels or post-run staining protocols is also possible. The buffer is optimized for highest specificity but may require additional fine-tuning of assay parameters like MgCl 2 concentration and annealing temperature.
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